Jamestown is a village in Bienville Parish, Louisiana, United States. The population was 139 at the 2010 census and 130 in 2017. Jamestown is 4 mi west of Kepler Lake. Jamestown is located in western Bienville Parish at 32°20′28″N 93°12′30″W / 32.34111°N 93.20833°W.