However, DNA polymerase cannot begin forming this new chain on its own and can only add nucleotides to a pre-existing 3'-OH group. A primer is therefore needed, at which nucleotides can be added.

A DNA isolation would not just be on your to-do list for the afternoon - it was the to-do list for the afternoon. However, in the mid-90s, "kits" started to become available. These were filled with ...

Mitochondrial DNA (mtDNA) polymerase γ (POLγ) harbours a 3′–5′ exonuclease proofreading activity. Here we demonstrate that this activity is required for the creation of ligatable ends ...

Almost all life on earth is based on DNA being copied, or replicated, and understanding how this process works could lead to a wide range of discoveries in biology and medicine. Now for the first time ...

To determine where DNA polymerase was and what it was doing, Xu and his colleagues combined two methods: confocal microscopy and optical tweezers. The team stretched an 8,000 kilobase strand of DNA ...

As well as assembling an entire mirror gene from L-DNA, the team has used mirror DNA to store a passage of text and conceal a hidden message within it (Nat. Biotechnol. 2021, DOI: 10.1038/s41587 ...

Although liquid biopsy has most often referred to the analysis of cfDNA from peripheral blood, this term also encompasses the isolation and analysis of tumor-derived material (e.g., DNA, RNA, or ...

Researchers reveal DNA repair mechanism Date: May 16, 2023 Source: NYU Langone Health / NYU Grossman School of Medicine Summary: A new study adds to an emerging, radically new picture of how ...

DNA polymerases are a group of enzymes that are essential to life due to their role in catalyzing DNA replication and repair. One such enzyme is Taq polymerase (Taq), which takes its name from Thermos ...

Many researchers have attempted to tackle this challenge, but they have had limited success. Recently, at the International Symposium on Human Identification (ISHI), scientists at Promega Corporation ...