Three-dimensional (3D) imaging is essential for investigating cellular structure and dynamics. Traditional optical methods ...

In the 20 th century, light microscopes relied solely on focusing light in space for the separation (=resolution) of adjacent tiny features. Either they focused the illumination light as sharply ...

Optical microscopes are still second to none when it comes to analyzing biological samples. However, their low resolution, improved only in recent years in STED microscopes, continues to be a problem.

A team has made a major breakthrough in the field of three-dimensional (3D) imaging of large-scale biological tissues. They ...

“The resolution of the standard optical microscope is basically limited by the diffraction barrier of light, which restricts the resolution to 200–300 nm for visible light,” explains Mark Schüttpelz, ...

The optical microscope, invented more than 400 years ago, has tended to grow larger and more complex as it has been modified to image ever-smaller objects with better resolution.

A new type of super-resolution optical microscopy takes a high-resolution image (at right) of graphite "nanoplatelets" about 100 nanometers wide. The imaging system, called saturated transient ...

For centuries diffraction limited the resolution of optical microscopy. The past 50 years have, however, seen one limitation after another buckle under the ingenuity of a host of wide-ranging ...

Tenfold improvement of lateral resolution up to 20nm: N-STORM utilizes high accuracy localization information for thousands of individual fluorophores present in a field of view to create breathtaking ...