Low Metabolic Burden Fermentation. Constitutive high plasmid copy number throughout the fermentation process is not necessary or desirable. Maintaining high copy numbers during biomass ...

Specifically, the overall plasmid size exceeds 7 kb, and commonly used Cas9 proteins are approximately /US/en160 kDa, significantly heightening the difficulty of in vivo delivery.

Visualization of plasmid size was facilitated by gel electrophoresis (Figure 2C). Plasmid DNA was effectively purified from both 0.5 mL and 1 mL LB and TB bacterial cultures, correspondingly.

At the Nevada Genomics Center we offer DNA sequencing using dye-terminator Sanger sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A Sanger sequencing reaction is run with ...

With it comes the expansion of its plasmid DNA (pDNA) capacity. The Seattle-based CDMO says that it can now produce hosts and plasmids with scales of 1L to 10L for high-quality pDNA.