The transfection-complex was formed by pipetting the same amount of plasmid dilution into the dilution of transfection reagent, followed by mixing and incubation for 30 min. Cell Culture and ...

B) Transfection: Plasmid DNA is diluted in 100 µL of commercially available, specialized transfection media or serum-free, antibiotic-free cell culture media. The amount of plasmid DNA used will ...

This plasmid includes the luciferase gene, allowing transformation efficiency to be assessed by photometric assay. ... Transfection of primary human macrophages with siRNA using Viromers.

An All-In-One Solution. Researchers looking for an all-in-one reagent to use across different experiments can choose the new X-tremeGENE™ 360 transfection reagent, a high-performing, versatile, and ...

Linearized plasmid DNA was purified by phenol/chloroform extraction and ethanol precipitation before being resuspended in TE buffer (10 m M Tris, 1 m M EDTA, pH 8.0) for transfection. Cell culture ...

This produces plasmid DNA in nuclease-free water, which can be used for a range of molecular biology methods, including transfection and cloning. Methods and results ...

Bacterial transformation was discovered in Streptococcus pneumoniae (S. pneumoniae) by Frederick Griffith in 1928. 3 He was conducting vaccination experiments in mice with S. pneumoniae bacteria and ...

An essential step in the development of almost any molecular therapeutic is the transfection of plasmids containing the therapeutic sequence into cells for delivery or production of the active ...