The transfection-complex was formed by pipetting the same amount of plasmid dilution into the dilution of transfection reagent, followed by mixing and incubation for 30 min.

High-Tech Polymers Leveraging Viral Biophysics Can Enhance the Transfection of Nucleic Acids Nucleic acid transfer is one of the central tools of the genomic era as it allows the manipulation of ...

In this article, we discuss the fundamentals of transfection, provide an overview of some of the commonly employed methods and a sample protocol. Finally, we consider some of the key applications of ...

Linearized plasmid DNA was purified by phenol/chloroform extraction and ethanol precipitation before being resuspended in TE buffer (10 m M Tris, 1 m M EDTA, pH 8.0) for transfection.

Researchers deliver transfected nucleic acids as oligonucleotides or in a viral or plasmid vector, which carries the genetic material into the host cells. Transfection can be stable or transient.

An essential step in the development of almost any molecular therapeutic is the transfection of plasmids containing the therapeutic sequence into cells for delivery or production of the active ...

In this article, we discuss transformation, bacterial transformation in particular, and how scientists can utilize it to manipulate bacteria for a plethora of purposes.

This produces plasmid DNA in nuclease-free water, which can be used for a range of molecular biology methods, including transfection and cloning. Methods and results ...