The genes of interest need to be cloned in a vector that is suitable for high-level constitutive protein expression in mammalian cells. In this protocol, the pHLsec plasmid is used 29,32.

(a) HEK293 cells were transiently transfected with a mixture of five plasmids, each containing an expression cassette for a single fluorescent protein or (b) a single MultiLabel plasmid containing ...

PROTEUS, a system designed to harness “directed evolution,” can speed the process up by years, or even decades.

Recombinant Protein expression has opened up avenues of research never before thought possible. However, ... (e.g. yeast, insect cells and mammalian cells, Figure 1), ...

Cornell researchers have found a new and potentially more accurate way to see what proteins are doing inside living ...

Researchers have developed a novel method for controlling the precise amount of protein produced by mammalian cells. A recent research effort from the Massachusetts Institute of Technology (MIT; MA, ...

Study: Control mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR. Image Credit: MMD Creative / Shutterstock.com. Current approaches to gene therapy. Gene control in ...

Professor Hirohide Saito (Department of Life Science Frontiers at CiRA / The University of Tokyo) and Assistant Professor ...

Producing a productive cell line for protein expression requires a series of steps, ... In 2024, WuXi Biologics launched its WuXia293, which is an HEK-based mammalian production system.

Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery. Science , 2021; 373 (6557): 882 DOI: 10.1126/science.abg6155 Cite This Page : ...

Tsai SF, Martin DI, Zon LI, D’Andrea AD, Wong GG, Orkin SH. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells. Nature 1989;339:446-451.