From our June 15 Issue: HPLC’s Discriminating Power Quietly Resolves Madding Mixtures and Methodically Sequesters Compounds When Waters introduced the first commercial high-performance liquid ...

We first evaluated the separation of a sample of trypsinized ribonuclease B using a Waters XBridge C18 column (3.5 µm, 4.6 mm × 100 mm) on each instrument where an obvious, but consistent ...

In this lab, students will determine the amount of caffeine in a beverage. Students will determine the retention time of pure caffeine. From this information, students will be able to identify the ...

HPLC alone cannot be used for identification, because different molecules can share so-called retention times. The retention time is the time taken for a solute to pass through the column.

LCGC International interviewed Bob Pirok from the University of Amsterdam, Netherlands to discuss strategies for enhancing ...

The efficiency of the separation process in reversed-phase HPLC is highly dependent on the binary hydro-organic mobile phase composition and surface properties of the packing material. ... RP HPLC ...

High performance liquid chromatography, more commonly known by the acronym HPLC, is a method used to identify substances in a mixture. There are several types of HPLC, such as reversed-phase ...

The retention time is measured under specific conditions and considered as the identifying characteristic of a given analyte. Sorbent particles might be hydrophobic or polar in nature. The commonly ...

For determination of selected carotenoids, various types of columns for high-performance liquid chromatography (HPLC) with different properties have been used. The characteristics of the ...