Incorporation of an internal ribosome entry site (IRES) into the gene therapy vector represents a promising strategy to efficiently co-express several gene products from the same promoter.

In contrast, ribosome-binding sites that are not substrates for the endogenous ribosome do not direct the synthesis of UPRT and survive the selection. The library is therefore selectively enriched ...

Through the application of protein cross-linking and high resolution mass spectrometry, we have detailed the ribosomal binding site of S1 and have observed evidence of its dynamics. Our results ...