Figure 2. Calibration curve of fluorescence intensity of five serial diluted reference solutions measured on a fluorescence spectrometer versus dye concentration. The intensity of one fluorescent bead ...

The calibration curve is thus fitted to an equation that relates pH to the intensity ratio between the sensor and reference fluorophores, which allows for application of the nanosensor.

A calibration curve of Rhodamine green is also shown. ... This makes FCS impossible because the main parameter of interest is not the fluorescence intensity itself but the deviation from the mean.