Using this approach we efficiently expressed a monomeric tandem mCherry-GFP-LC3 construct in neurons of the peripheral and central nervous system, allowing the measurement of autophagy activity in ...

To investigate whether seipin is directly related to the autophagy system, we co-transfected wild-type seipin and two seipin mutants with the autophagosome marker GFP-LC3 into HEK-293 cells.

With the help of several autophagy-related (ATG) proteins, a small, ubiquitin-like protein called LC3 (LC3-II when bound) binds the nascent phagophore to direct its expansion around cytoplasmic ...

We found that lipidated LC3 was recruited by lysosomes in response to damage, and that the lipidated protein interacted with TRPML1, causing increased calcium efflux from the lysosome, which ...

The roles of key proteins such as the microtubule-associated protein 1 light chain 3 (LC3), beclin 1, and p62 in autophagy initiation and substrate recognition were also explored.

Autophagy is defined as regulated self-eating that occurs through the degradation of cellular components. It is an important response to cellular starvation.

The 2016 Nobel Prize in Physiology or Medicine has been awarded to Yoshinori Ohsumi for his discoveries of mechanisms for autophagy -- a fundamental process for degrading and recycling cellular ...

In the immune system, autophagy is involved in B cell self-renewal and the survival of memory and plasma B cells. However, the effects of enhancing autophagy in B cells are not well understood.

A study found the failure of the brain neuronal cells’ normal house-cleaning function may be causing a buildup of degraded proteins in Parkinson's disease.