Join us for an expert-led webinar with Adham Ahmed as he delves into the intricacies of developing robust and reliable reversed-phase chromatography methods. This session will cover critical ...

Reversed-phase LC separation of tryptic peptides of Herceptin and a biosimilar on an Agilent AdvanceBio Peptide mapping column, 2.1 x 100 mm, 2.7 µm column. Biopharma and LC Solutions ...

Many reversed-phase columns advertised for such separations harbor small pores which restrict separation of dsDNA fragments over ... Fragments Using a Wide-Pore Reversed-Phase Chromatography Column.

Ion pair reversed phase chromatography is carried out on non-polar “reversed phase” columns by adding ion pairing reagents to the mobile phase. For instance, trifluoroacetic acid is used for pairing ...

We now find that satisfactory reversed-phase columns may be prepared by the following procedure. Chloroform (8 c.c.) is shaken thoroughly with ‘Pyrex’ glass (80 gm.) in No. 100 powder.

Liquid-liquid chromatography can be divided into normal-phase chromatography (NPC) and reverse-phase chromatography (RPC) according to the types of stationary and mobile phases. With the rapid ...

In the case of reversed phase chromatography, less polar analytes will preferentially partition into the non-polar stationary phase and have a longer retention time. The data acquisition system ...

In traditional Reverse-phase Liquid Chromatography (RPLC), polar, hydrophilic and charged compounds are preferentially retained on the column.

Some of the methods utilized include size exclusion chromatography for the quantitation of dimers as well as ... "Oxidized insulin chain assessment using Reversed Phase Agilent ZORBAX RRHD 300SB ...

Using supercritical fluid chromatography (SFC) (4) in place of traditional reversed-phase and normal-phase high-performance liquid chromatography can greatly reduce the timelines for this process.