Transfection efficiency depends on a number of factors. “For most adherent cells it is standard to split them the day prior so they will be ~ 80% confluent at the time of transfection.

This article highlights the current state of macromolecular gene delivery systems, their applications, and the ongoing ...

Following the direct injection of plasmid DNA expressing a reporter gene into skeletal muscle, both age and sex of the rodents have been shown to play an important role in transfection efficiency ...

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The ratio of plasmid DNA to transfection agent within polyplexes had a major impact on transfection efficiency. Therefore, each expression vector needs a fine tuning of transfection conditions to ...

B) Transfection: Plasmid DNA is diluted in 100 µL of commercially available, specialized transfection media or serum-free, antibiotic-free cell culture media. The amount of plasmid DNA used will ...

Iwuchukwu elaborates, “These cells are transfected with either triple or dual plasmid transfection systems, producing the vector of choice. The HEK293 cells are then lysed, and the harvest ...

A novel cationic nanotechnology called jetOPTIMUS ® was created to increase the effectiveness of DNA transfection in adherent cells, including difficult-to-transfect cells. Transfection with ...

Gene therapy has emerged as a revolutionary approach to treating genetic disorders by directly addressing underlying genetic abnormalities. However ...

"The modRNA was found to be notably more efficient than plasmid DNA," Lian said. "Around 90% of the cells received the modRNA from a simple transfection, so it was able to remain in place and do ...

Silica membrane-based DNA purification kits are a convenient way to prepare high quality, transfection-grade plasmid DNA samples for cloning, sequencing and restriction analysis or for more sensitive ...