In this example, we were able to transfer a complex peptide mapping method from an HPLC instrument and achieve nearly identical chromatography on a UPLC instrument by making one simple ...

For example, on HPLC columns, it is not possible to separate most ionic analytes. This means that HPLC is not suitable for analyses of standard cations or anions in various foods and water.

Equation 1 and Figure 4: HPLC chromatograms of (a) progesterone reference standard; (b) separation of progesterone gel sample; (c) placebo formulation. Experimental Chemicals and reagents. All ...

Please use one of the following formats to cite this article in your essay, paper or report: APA. Sartorius. (2023, June 09). Why ultrapure water is critical for HPLC analysis.

RP-HPLC can also be used to study the encapsulation and release of active ingredients in nanoparticles. For example, the RP-HPLC method with 0.1% trifluoroacetic acid as a mobile phase can be used to ...

9. R.W. Stringham, "Chiral Separation of Amines in Subcritical Fluid Chromatography using Polysaccharide Stationary Phases and Acidic Additives," J Chromatogr. A, 1070, 163 (2005). 10. E Francotte, ...