"The hook effect occurs when the HCG concentration is so high that it saturates both antibodies and there are so many molecules that the antibodies don't actually form a sandwich.

Prozone Effect One major limitation is the "prozone effect" or "hook effect," where high levels of antibodies inhibit the binding of the reporter antibody, resulting in falsely low signals.

In a significant step toward improving targeted cancer therapy, researchers from Japan have developed a novel nanocarrier ...

This high-dose hook effect has been documented with immunoassays for β-hCG, ferritin, prostate-specific antigen, prolactin, thyrotropin, and CA-125. 5 These inaccurate results can be overcome by ...

Technically, you can have a hook effect with any kind of medical lab test: blood, urine, and saliva. The hook effect will give you a false negative, when you should have a positive result.

Is it possible to improve the antibodies that the body produces to fight SARS-CoV2? In a study led by researchers from Lund University in Sweden, this was investigated by redesigning antibodies ...

Dilution did not demonstrate interference or hook effect (a falsely low test result because of an excess of antigen in a sandwich immunoassay).

TPOAb and TgAb IMAs should be checked for hook effects using ~20 specimens with antibody concentrations >1,000 kIU/L and ~20 specimens with values above 10,000 kIU/L.

However, if you’ve already got too much hCG in your body, the antibodies will be flooded and the test will come back negative. This is called the ‘hook effect’.

While most CD73 antibodies in the clinic frequently experienced the "hook effect", where the antibody loses its effectiveness at high concentrations, uliledlimab is designed to unhook this affect ...