The design and optimization of a surface plasmon resonance (SPR) biosensor for very sensitive detection of Mycobacterium tuberculosis are presented in this work. Using the transfer matrix method (TMM) ...

Induced electrocompetent Mycobacterium smegmatis pJV53 cells are co-electroporated with phage DNA (50–100 ng) and a 200 bp double-stranded DNA recombineering substrate (50–500 ng), mixed with ...